The integration misalignment of INS/DVL is an important factor to affect the positioning accuracy of integrated navigation system. How to accurately estimate the misalignment angle between the two equipments is the key to improve the positioning accuracy of integrated navigation system. Misalignment angle estimation based on the speed of fitting the observed speed high accuracy requirements, it is estimated that the accuracy is affected by measurement noise, while enabling more complex process .To solve these problems, using an estimation method based on the sail track, using singular value decomposition (SVD) least squares algorithm to achieve the online estimate of the misalignment angle. Simulation results show that, in the case of the GPS positioning error of 20 meters, the three estimate errors of 50 Monte Carlo simulations is 0.1001°, 0.0283°, 0.0213°. This method is simply, high estimate accuracy and having good practical value.