As an important component of civil aircraft requirements, safety-related requirements are closely related to the safety assessment process. The safety assessment process of civil aircraft is introduced, and safety-related requirements of avonics for a certain system configuration is analyzed, which starts from the function hazard analysis and utilities safety analysis methods such as Fault Tree Analysis (FTA); the deduced safety-related requirements include probabilistic item availability requirements for hardware failure, Development Assurance Level (DAL) requirements for mitigating design errors, and other derived requirements. The analysis method of safety-related requirements complies with SAE ARP 4754A, SAE ARP 4761 and other design guidelines, and has important reference significance for the requirements development and validation of airborne and ground equipment of civil aircraft with high safety targets.